/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-VCIP135 Antibody
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Primary Antibodies  

Rabbit anti-VCIP135 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

VCIP135

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1172 and 1222

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

VCIP135 (valosin-containing protein p97/p47 complex-interacting protein p135) acts as a deubiquitinating enzyme. VCIP135 is necessary for VCP-mediated reassembly of Golgi stacks after mitosis and may play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). VCIP135 also mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q96JH7].

80124

VCPIP1

valosin containing protein interacting protein 1

Q96JH7

Deubiquitinating protein VCIP135

Alternate Names

deubiquitinating protein VCIP135; DUBA3; valosin-containing protein p97/p47 complex-interacting protein 1; valosin-containing protein p97/p47 complex-interacting protein p135; VCIP135; VCP/p47 complex-interacting 135-kDa protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.