/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-UCH-L3 Antibody
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Primary Antibodies  

Rabbit anti-UCH-L3 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

UCH-L3

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 174 and 224

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Pig,

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Ubiquitin carboxyl-terminal hydrolase isoenzyme 3 (UCH-L3) is a deubiquitinating enzyme (DUB) and thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P15374].

7347

UCHL3

ubiquitin C-terminal hydrolase L3

P15374

Ubiquitin carboxyl-terminal hydrolase isozyme L3

Alternate Names

testicular tissue protein Li 221; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.