/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MMS21 Antibody
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Primary Antibodies  

Rabbit anti-MMS21 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

MMS21

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 197 and 247

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The human MMS21 (Methyl Methanesulfonate-Sensitivity protein 21) homolog is also known as E3 SUMO-protein ligase NSE2. MMS21 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96MF7].

286053

NSMCE2

NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase

Q96MF7

E3 SUMO-protein ligase NSE2

Alternate Names

C8orf36; E3 SUMO-protein ligase NSE2; E3 SUMO-protein transferase NSE2; hMMS21; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; Non-SMC element 2 homolog; non-SMC element 2, MMS21 homolog; non-structural maintenance of chromosomes element 2 homolog; NSE2; NSMCE2/PVT1 fusion; PVT1/NSMCE2 fusion; zinc finger, MIZ-type containing 7; ZMIZ7

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.