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Primary Antibodies  

Rabbit anti-MGEA5 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

MGEA5

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 500 and 550

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].

10724

OGA

O-GlcNAcase

O60502

Protein O-GlcNAcase

Alternate Names

beta-hexosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; MEA5; meningioma expressed antigen 5 (hyaluronidase); meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; protein O-GlcNAcase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.