/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Kindlin-2 Antibody
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Primary Antibodies  

Rabbit anti-Kindlin-2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Kindlin-2

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Kindlin-2 is a scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. It binds to membranes enriched in phosphoinositides. It also enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Kindlin-2 is required for the assembly of focal adhesions and participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Kindlin-2 recruits FBLIM1 to focal adhesions. It plays a role in the TGFB1 and integrin signaling pathways, and it stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96AC1].

10979

FERMT2

fermitin family member 2

Q96AC1

Fermitin family homolog 2

Alternate Names

fermitin family homolog 2; KIND2; kindlin 2; Kindlin-2; MIG2; mig-2; mitogen inducible gene 2 protein; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain containing, family C member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112; UNC112B

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.