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Primary Antibodies  

Rabbit anti-Aspartyl-tRNA Synthetase/DARS Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

Aspartyl-tRNA Synthetase/DARS

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 25 and 75

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Aspartyl-tRNA Synthetase/DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14868].

1615

DARS

aspartyl-tRNA synthetase

P14868

Aspartate--tRNA ligase, cytoplasmic

Alternate Names

aspartate tRNA ligase 1, cytoplasmic; aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; aspartyl-tRNA synthetase, cytoplasmic; aspRS; cell proliferation-inducing gene 40 protein; HBSL; testicular tissue protein Li 192

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.