/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SF3A2/SAP62 Antibody
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Primary Antibodies  

Rabbit anti-SF3A2/SAP62 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SF3A2/SAP62

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Bovine,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

SF3A2 is subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein [taken from NCBI Entrez Gene (Gene ID: 8175)].

8175

SF3A2

splicing factor 3a subunit 2

Q15428

Splicing factor 3A subunit 2

Alternate Names

pre-mRNA splicing factor SF3A, subunit 2; PRP11; PRPF11; SAP 62; SAP62; SF3a66; spliceosome associated protein 62; Spliceosome-associated protein 62; splicing factor 3A subunit 2; splicing factor 3a, subunit 2, 66kD; splicing factor 3a, subunit 2, 66kDa

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.