/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MPRIP/M-RIP Antibody
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Primary Antibodies  

Rabbit anti-MPRIP/M-RIP Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

MPRIP/M-RIP

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 550 and 600

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

MPRIP targets myosin phosphatase to the actin cytoskeleton. It is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MPRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6WCQ1].

23164

MPRIP

myosin phosphatase Rho interacting protein

Q6WCQ1

Myosin phosphatase Rho-interacting protein

Alternate Names

MRIP; M-RIP; myosin phosphatase Rho-interacting protein; p116Rip; Rho interacting protein 3; Rho-interacting protein 3; RHOIP3; RIP3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.