/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-POLR1E/PRAF1 Antibody
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Primary Antibodies  

Rabbit anti-POLR1E/PRAF1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

POLR1E/PRAF1

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 431 and 481

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

POLR1E is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase I which synthesizes ribosomal RNA precursors and appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9GZS1].

64425

POLR1E

RNA polymerase I subunit E

Q9GZS1

DNA-directed RNA polymerase I subunit RPA49

Alternate Names

DNA-directed RNA polymerase I subunit E; DNA-directed RNA polymerase I subunit RPA49; PAF53; polymerase (RNA) I associated factor 1; polymerase (RNA) I polypeptide E, 53kDa; polymerase (RNA) I subunit E; PRAF1; RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.