/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-PSMD5/26S Proteasome Subunit S5B Antibody
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Primary Antibodies  

Rabbit anti-PSMD5/26S Proteasome Subunit S5B Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

PSMD5/26S Proteasome Subunit S5B

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 325 and 375

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

PSMD5/26S Proteasome Subunit S5B acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16401].

5711

PSMD5

proteasome 26S subunit, non-ATPase 5

Q16401

26S proteasome non-ATPase regulatory subunit 5

Alternate Names

26S protease subunit S5 basic; 26S proteasome non-ATPase regulatory subunit 5; 26S proteasome subunit S5B; proteasome (prosome, macropain) 26S subunit, non-ATPase, 5; S5B

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.