/ ANTIBODIES/ANTISERA / MAPK Pathway Antibodies / Rabbit anti-PLA2G4A/cPLA2 Antibody
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MAPK Pathway Antibodies  

Rabbit anti-PLA2G4A/cPLA2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

PLA2G4A/cPLA2

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 699 and 749

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The gene PLA2G4A encodes a member of the cytosolic phospholipase A2 group IV family, cytosolic phospholipase A2. This enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate inflammatory responses. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor [taken from NCBI Entrez Gene (Gene ID: 5321)].

5321

PLA2G4A

phospholipase A2 group IVA

P47712

Cytosolic phospholipase A2

Alternate Names

calcium-dependent phospholipid-binding protein; cPLA2; cPLA2-alpha; cytosolic phospholipase A2; lysophospholipase; phosphatidylcholine 2-acylhydrolase; Phospholipase A2; Phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.