/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-LCP2/SLP76 Antibody
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Primary Antibodies  

Rabbit anti-LCP2/SLP76 Antibody Affinity Purified

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

LCP2/SLP76

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 225 and 275

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Lymphocyte cytosolic protein 2 (LCP2) was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. LCP2 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with LCP2 both constitutively and inducibly following receptor ligation, supporting the notion that LCP2 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that LCP2 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function [taken from NCBI Entrez Gene (Gene ID: 3937)].

3937

LCP2

lymphocyte cytosolic protein 2

Q13094

Lymphocyte cytosolic protein 2

Alternate Names

76 kDa tyrosine phosphoprotein; lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP76; SLP-76; SLP-76 tyrosine phosphoprotein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.