/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-IST1/OLC1 Antibody
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Primary Antibodies  

Rabbit anti-IST1/OLC1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

IST1/OLC1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 300 and 350

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Increased sodium tolerance 1 homolog (IST1) is a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis [taken from NCBI Entrez Gene (Gene ID: 9798)].

9798

IST1

IST1, ESCRT-III associated factor

P53990

IST1 homolog

Alternate Names

hIST1; increased sodium tolerance 1 homolog; IST1 homolog; IST1, endosomal sorting complex required for transport-III component; OLC1; overexpressed in lung cancer 1; putative MAPK-activating protein PM28

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.