/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HMCES Antibody
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Primary Antibodies  

Rabbit anti-HMCES Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

HMCES

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 300 and 350

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

HMCES specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells, suggesting that it acts as a specific reader of 5hmC in stem cells (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96FZ2].

56941

HMCES

5-hydroxymethylcytosine binding, ES cell specific

Q96FZ2

Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein

Alternate Names

5-hydroxymethylcytosine (hmC) binding, ES cell-specific; C3orf37; DC12; embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein; ES cell-specific 5hmC-binding protein; putative peptidase SRAPD1; SOS response associated peptidase domain containing 1; SRAP domain-containing protein 1; SRAPD1; UPF0361 protein C3orf37

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.