/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ORP150/HYOU1 Antibody, Affinity Purified
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Primary Antibodies  

Rabbit anti-ORP150/HYOU1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ORP150/HYOU1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 949 and 999

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

ORP150 belongs to the heat shock protein 70 family that is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. ORP150 is also thought to play an important role in protein folding and secretion in the ER and is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. Finally, ORP150 has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. [taken from NCBI Entrez Gene (Gene ID: 10525)].

10525

HYOU1

hypoxia up-regulated 1

Q9Y4L1

Hypoxia up-regulated protein 1

Alternate Names

150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; epididymis secretory sperm binding protein; Grp170; GRP-170; HSP12A; hypoxia up-regulated protein 1; ORP150; ORP-150; oxygen regulated protein (150kD)

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.