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Goat anti-Dog IgM Heavy Chain Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
Target:
Dog IgM Heavy Chain
Reactivity:
Dog
Applications:
DD
,ELISA
,ICC
,IEP
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
,Antiserum
Antiserum (2 ml)
Unconjugated (1 mg)
FITC (1 mg)
HRP (1 mg)
Product Details
Specifications
Verified Reactivity
Dog
Antigen Species
Dog
Concentration
1 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Serum containing 0.09% Sodium Azide
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Production Procedures
Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with dog IgM. No antibody was detected against immunoglobulin light chains or non-immunoglobulin serum proteins. This antibody may cross react with IgM from other species.
Additional Product Information
IgM is found primarily in serum and is the first antibody made to fight infections. Anti-heavy chain antibodies are designed to react with only the heavy chain of the Ig molecule and remove reactivity to the light chain.
Applications
