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Donkey anti-Goat IgG Heavy and Light Chain Antibody Biotinylated

bethyl
Bethyl Laboratories

Catalog #

Target:

Goat IgG Heavy and Light Chain

Reactivity:

Goat

Host:

Donkey

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Biotin

,

DyLight® 488

,

DyLight® 550

,

DyLight® 594

,

DyLight® 650

,

FITC

,

HRP

,

Unconjugated

Purity:

Antigen Affinity Purified

,

Antiserum

Antiserum (2 ml)

Unconjugated (1 mg)

Biotin (1 mg)

DyLight® 488 (0.5 mg)

DyLight® 550 (0.5 mg)

DyLight® 594 (0.5 mg)

DyLight® 650 (0.5 mg)

FITC (1 mg)

HRP (1 mg)

Product Details

Specifications
Verified Reactivity

Goat

Antigen Species

Goat

Concentration
1 mg/ml 0.5 mg/ml
Fluorophore/Protein
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt,

2 years from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


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Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400


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Serum containing 0.09% Sodium Azide


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Production Procedures

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

This antibody may cross react with IgG from other species.

Additional Product Information

Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.

Applications

Not all listed applications have been specifically tested by our laboratory. For use with fixed Paraffin-Embedded tissues and cells and fresh- frozen or fixed tissue and cells. For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).

Optimal working dilutions should be determined experimentally by the investigator. DyLight® 488 is excited at 493 (in PBS) and emits at 518 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 550 is excited at 562 (in PBS) and emits at 576 (in PBS). DyLight® 550 replaces DyLight® 549.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 594 is excited at 593 (in PBS) and emits at 618 (in PBS).

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. DyLight® 650 is excited at 652 (in PBS) and emits at 672 (in PBS). DyLight® 650 replaces DyLight® 649.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.