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Rabbit anti-Goat IgG-Fc Fragment Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Goat IgG-Fc Fragment
Reactivity:
Goat
Applications:
ELISA
,Flow Cyt
,ICC
,IF
,IHC
,WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,DyLight® 488
,DyLight® 550
,DyLight® 594
,DyLight® 650
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
DyLight® 488 (0.5 mg)
DyLight® 550 (0.5 mg)
DyLight® 594 (0.5 mg)
DyLight® 650 (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
Product Details
Specifications
Verified Reactivity
Goat
Antigen Species
Goat
Minimum Reactivity Species
Chicken, Horse, Human, Mouse, Pig, Rat
Concentration
0.5 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG-Fc Fragment. Less than 1% cross reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG-Fc Fragment. Less than 1% cross reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG-Fc Fragment. Less than 1% cross reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG-Fc Fragment. Less than 1% cross reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG-Fc Fragment. Less than 1% cross reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
This antibody may cross react with IgG from other species.
Additional Product Information
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Applications
