/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]
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Primary Antibodies  

Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

NAMPT/PBEF/Visfatin

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR058F

Format:

Whole IgG

Immunogen:

between 400 and 450

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
250 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 400 and 450 of human Nicotinamide phosphoribosyltransferase precursor (NAMPT), using the numbering given in entry NP_005737.1 (Gene ID 10135).

Additional Product Information

PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.

10135

NAMPT

nicotinamide phosphoribosyltransferase

P43490

Nicotinamide phosphoribosyltransferase

Alternate Names

1110035O14Rik; NAmPRTase; Nampt; nicotinamide phosphoribosyltransferase; PBEF; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VF; VISFATIN

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.