/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H]
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Primary Antibodies  

Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody [BLR101H]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

CDK1 Phospho (Y15)

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR101H

Format:

Whole IgG

Immunogen:

surrounding tyrosine 15

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
40 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Cyclin-dependent kinase 1 (CDK1) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CKD1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control [taken from NCBI Entrez Gene (Gene ID: 983)].

983

CDK1

cyclin dependent kinase 1

P06493

Cyclin-dependent kinase 1

Alternate Names

CDC2; CDC28A; CDK1; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; cyclin-dependent kinase 1; p34 protein kinase; P34CDC2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.