/ ANTIBODIES/ANTISERA / MAPK Pathway Antibodies / Rabbit anti-CD14 Recombinant Monoclonal Antibody [BLR187J]
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Rabbit anti-CD14 Recombinant Monoclonal Antibody [BLR187J]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CD14

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Platforms:

COMET™

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR187J

Format:

Whole IgG

Immunogen:

residues 20-352 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
50 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of human monocyte differentiation antigen CD14 (residues 20-352, NP_000582.1).

Additional Product Information

CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. [taken from NCBI Entrez Gene (Gene ID: 929)].

929

CD14

CD14 molecule

P08571

Monocyte differentiation antigen CD14

Alternate Names

CD antigen CD14; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.