/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-F4/80 Recombinant Monoclonal Antibody [BLR209K]
Primary Antibodies  

Rabbit anti-F4/80 Recombinant Monoclonal Antibody [BLR209K]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

F4/80

Reactivity:

Mouse

Applications:

ICC

,

IHC

,

IP

,

WB

Platforms:

COMET™

,

PhenoImager™ HT

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR209K

Format:

Whole IgG

Immunogen:

Residues 28-225 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Additional Product Information

Adhesion G protein-coupled receptor E1 is also known as F4/80. It is an orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. In mouse, F4/80 is a well-characterized and extensively referenced macrophage marker. It is also known as Ly71 and EMR1, and is part of the EGF-TM7 family. It is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).

13733

Adgre1

adhesion G protein-coupled receptor E1

Q61549

Adhesion G protein-coupled receptor E1

Alternate Names

adhesion G protein-coupled receptor E1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; TM7LN3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.