/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR214K]
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Primary Antibodies  

Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR214K]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CD22

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR214K

Format:

Whole IgG

Immunogen:

residues 20-687 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
500 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of human B-cell receptor CD22 (residues 20-687, NP_001762.2).

Additional Product Information

CD22 mediates B-cell B-cell interactions and is involved in the localization of B-cells in lymphoid tissues. CD22 binds sialylated glycoproteins including CD45. It is involved in B cell activation; negative regulation of the B cell receptor signaling pathway; and regulation of endocytosis. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20273].

933

CD22

CD22 molecule

P20273

B-cell receptor CD22

Alternate Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD antigen CD22; CD22 antigen; sialic acid-binding Ig-like lectin 2; SIGLEC2; SIGLEC-2; T-cell surface antigen Leu-14

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.