/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-GLI1 Recombinant Monoclonal Antibody [BLR256L]
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Primary Antibodies  

Rabbit anti-GLI1 Recombinant Monoclonal Antibody [BLR256L]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

GLI1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR256L

Format:

Whole IgG

Immunogen:

between 1056 and 1106 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
100 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

GLI1, a member of the Kruppel family of zinc finger proteins, is a transcription factor activated by the sonic hedgehog signal transduction cascade that regulates stem cell proliferation. The activity and nuclear localization of GLI1 is negatively regulated by p53 in an inhibitory loop. [taken from NCBI Entrez Gene (Gene ID: 2735)].

2735

GLI1

GLI family zinc finger 1

P08151

Zinc finger protein GLI1

Alternate Names

GLI; GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); oncogene GLI; PAPA8; zinc finger protein GLI1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.