/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody [BLR268L]
Primary Antibodies  

Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody [BLR268L]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

P-Selectin/CD62P

Reactivity:

Human

,

Mouse

Applications:

flow_cyt

,

ICC

,

IHC

,

IP

,

mIF

,

WB

Platforms:

COMET™

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR268L

Format:

Whole IgG

Immunogen:

residues 42-709 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of mouse P-Selectin/CD62P (residues 42-709, NP_035477.1).

Additional Product Information

P-Selectin, or CD62P, is a Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. It mediates the interaction of activated endothelial cells or platelets with leukocytes. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P16109].

20344

Selp

selectin, platelet

Q01102

P-selectin

Alternate Names

CD antigen CD62P;CD62 antigen-like family member P;CD62P;GMP-140;granule membrane protein 140;Grmp;LECAM3;leukocyte-endothelial cell adhesion molecule 3;PADGEM;platelet activation dependent granule-external membrane protein;P-selectin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.