/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody [BLR295L]
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Primary Antibodies  

Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody [BLR295L]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

E-Selectin/CD62E

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR295L

Format:

Whole IgG

Immunogen:

residues 22-555 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of human E-selectin (residues 22-555, NP_000441.2).

Additional Product Information

E-Selectin, aka CD62E, is a cell-surface glycoprotein with a role in immunoadhesion. It is responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining, acting via interaction with SELPLG/PSGL1. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P16581].

6401

SELE

selectin E

P16581

E-selectin

Alternate Names

CD antigen CD62E;CD62 antigen-like family member E;CD62E;ELAM;ELAM1;ELAM-1;endothelial adhesion molecule 1;endothelial leukocyte adhesion molecule 1;ESEL;E-selectin;LECAM2;leukocyte endothelial cell adhesion molecule 2;Leukocyte-endothelial cell adhesion molecule 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.