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Goat anti-Human Lambda Light Chain Antibody Biotinylated

bethyl
Bethyl Laboratories

Catalog #

Target:

Human Lambda Light Chain

Reactivity:

Human

Host:

Goat

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Biotin

,

FITC

,

HRP

,

Unconjugated

Purity:

Antigen Affinity Purified

,

IgG Fraction

IgG Fraction (2 ml)

Unconjugated (1 mg)

Biotin (1 mg)

FITC (1 mg)

HRP (1 mg)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1 mg/ml
Fluorophore/Protein
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt,

2 years from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide


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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400


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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


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Production Procedures

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.

By immunoelectrophoresis and ELISA this antibody reacts specifically with lambda light chains common to all human immunoglobulins. No antibody was detected against kappa or non-immunoglobulin serum proteins. This antibody may cross react with lambda light chain from other species.

Additional Product Information

The lambda light chain is a component of an Ig molecule that typically forms an Ig molecule linking to a heavy chain. The human body makes both kappa and lambda light chains, and elevated lambda levels may suggest disorders such as amyloidosis or multiple myeloma.

Applications

Not all listed applications have been specifically tested by our laboratory. For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).

Optimal working dilutions should be determined experimentally by the investigator.