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Goat anti-Mouse IgG2c Heavy Chain Antibody Biotinylated
Bethyl Laboratories
Catalog #
Target:
Mouse IgG2c Heavy Chain
Reactivity:
Mouse
Applications:
ELISA
,ICC
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (1 mg)
Biotin (1 mg)
FITC (1 mg)
HRP (1 mg)
Product Details
Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Concentration
1 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c; sometimes referred to as the Igh 1b allele of IgG2a. (Martin et al., Journal of Immunological Methods, 1998, 212, 187-192) The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to biotin.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunoglobulin serum proteins. This antibody may cross react with IgG2c from other species.
Biotinylated antibody was demonstrated by reaction with avidin/peroxidase when coated on microtiter wells. Working dilutions should be determined by the investigator.
Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c; sometimes referred to as the Igh 1b allele of IgG2a. (Martin et al., Journal of Immunological Methods, 1998, 212, 187-192) The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunoglobulin serum proteins.
This antibody may cross react with IgG2c from other species.
Additional Product Information
Mouse IgG2c is generally regarded as the most active IgG subclass, as it can interact with all mouse activating Fc receptors.
Applications
