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Goat anti-Mouse IgG1 Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Mouse IgG1
Reactivity:
Mouse
Applications:
ELISA
,Flow Cyt
,ICC
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
Product Details
Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Minimum Reactivity Species
Human, Rat
Concentration
0.5 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Production Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG1 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG1 was detected. This antibody may cross react with IgG1 from other species.
Additional Product Information
IgG1 is the most dominant subclass of IgG and is known to be able to cross the placenta, bind strongly to Fc receptors, and activate the complement pathway efficiently.
Applications
