NEW!
Goat anti-Mouse IgG2a Cross-Adsorbed Antibody FITC Conjugated
Bethyl Laboratories
Catalog #
Target:
Mouse IgG2a
Reactivity:
Mouse
Applications:
ELISA
,Flow Cyt
,ICC
,IF
,IHC
,WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Biotin
,FITC
,HRP
,Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)
Biotin (0.5 mg)
FITC (0.5 mg)
HRP (0.5 mg)
Product Details
Specifications
Verified Reactivity
Mouse
Antigen Species
Mouse
Minimum Reactivity Species
Human, Rat
Concentration
0.5 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
Request Formulation Change
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was solid phase adsorbed to ensure subclass specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG2a was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2a. Cross reactivity with immunoglobulins is less than 2%. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG2a was detected. This antibody may cross react with IgG2a from other species.
Additional Product Information
IgG2 is the second more prevalent subclass of IgG. IgG2a is superior to IgG1 in activating complement and interacts very strongly with FcγR.
Applications
