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Rabbit anti-ILK IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ILK

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 402 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Guinea Pig,

Mouse,

Horse,

Sheep,

Dog,

Chicken,

Pig,

Rabbit,

Zebrafish,

Bovine,

Monkey,

Rat

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ILK immobilized on solid support.

The epitope recognized by IHC-00361 maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ILK immobilized on solid support.

The epitope recognized by IHC-00361-T maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.

3611

ILK

integrin linked kinase

Q13418

Integrin-linked protein kinase

Alternate Names

59 kDa serine/threonine-protein kinase; epididymis secretory protein Li 28; HEL-S-28; ILK-1; ILK-2; integrin-linked kinase-2; integrin-linked protein kinase; P59; p59ILK

Applications

Acetone-Fixed Cells 1:100 - 1:500:

Formaldehyde fixation is not recommended.