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Rabbit anti-WTAP IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

WTAP

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 346 and 396

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Guinea Pig,

Horse,

Pig,

X. laevis,

X. tropicalis,

Bovine,

Monkey

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.

The epitope recognized by IHC-00368 maps to a region between residue 346 and 396 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.

The epitope recognized by IHC-00368-T maps to a region between residue 346 and 396 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

Wilms’ tumor 1 associated protein (WTAP) is a protein found to associate with the Wilms’ tumor suppressor (WT1). WTAP appears to play a role in cell proliferation and regulation of the G2/M transition. WTAP has been suggested to influence these processes via a function in mRNA stabilization and mRNA splicing.

9589

WTAP

WT1 associated protein

Q15007

Pre-mRNA-splicing regulator WTAP

Alternate Names

female-lethal(2)D homolog; hFL(2)D; Mum2; PNAS-132; pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1-associated protein

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.