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Rabbit anti-CKAP2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CKAP2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 375 and 425
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CKAP2 immobilized on solid support.

The epitope recognized by A302-150A-T maps to a region between residue 375 and 425 of human cytoskeleton associated protein 2 using the numbering given in entry NP_001091995.1 (GeneID 26586).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CKAP2 immobilized on solid support.

The epitope recognized by A302-150A maps to a region between residue 375 and 425 of human cytoskeleton associated protein 2 using the numbering given in entry NP_001091995.1 (GeneID 26586).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

CKAP2 (cytoskeletal-associated protein 2), also known as TMAP (tumor-associated microtubule-associated protein), was identified as an mRNA up-regulated in primary human gastric cancers. It was found to localize to microtubule organizing centers and microtubules and play a role in cell division. CKAP2 is required for proper chromosome segregation and the maintenance of genomic stability. It has recently been shown to be a substrate of Cdk1-cyclinB and essential for bipolar spindle formation.

26586
CKAP2
cytoskeleton associated protein 2
Q8WWK9
Cytoskeleton-associated protein 2

Alternate Names

CTCL tumor antigen se20-10; cytoskeleton-associated protein 2; LB1; se20-10; TMAP; tumor- and microtubule-associated protein; tumor-associated microtubule-associated protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.