Rabbit anti-CKAP2 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A302-150A-T maps to a region between residue 375 and 425 of human cytoskeleton associated protein 2 using the numbering given in entry NP_001091995.1 (GeneID 26586).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CKAP2 immobilized on solid support.
The epitope recognized by A302-150A maps to a region between residue 375 and 425 of human cytoskeleton associated protein 2 using the numbering given in entry NP_001091995.1 (GeneID 26586).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CKAP2 (cytoskeletal-associated protein 2), also known as TMAP (tumor-associated microtubule-associated protein), was identified as an mRNA up-regulated in primary human gastric cancers. It was found to localize to microtubule organizing centers and microtubules and play a role in cell division. CKAP2 is required for proper chromosome segregation and the maintenance of genomic stability. It has recently been shown to be a substrate of Cdk1-cyclinB and essential for bipolar spindle formation.
Alternate Names
CTCL tumor antigen se20-10; cytoskeleton-associated protein 2; LB1; se20-10; TMAP; tumor- and microtubule-associated protein; tumor-associated microtubule-associated protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.