/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Cul2 Antibody
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Primary Antibodies  

Rabbit anti-Cul2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (8)
Target:

Cul2

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 695 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Cul2 immobilized on solid support.

The epitope recognized by A302-476A maps to a region between residue 695 and 745 of human cullin 2 using the numbering given in entry NP_003582.2 (GeneID 8453).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to Cul2 immobilized on solid support.

The epitope recognized by A302-476A-T maps to a region between residue 695 and 745 of human cullin 2 using the numbering given in entry NP_003582.2 (GeneID 8453).

Country of Origin

USA

Additional Product Information

Cullin-2 (Cul2) is a core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. Cul2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13617].

8453

CUL2

cullin 2

Q13617

Cullin-2

Alternate Names

CUL-2; cullin-2; testis secretory sperm-binding protein Li 238E

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.