/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SCYL2 Antibody
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Primary Antibodies  

Rabbit anti-SCYL2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

SCYL2

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 850 and 900

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to SCYL2 immobilized on solid support.

The epitope recognized by A304-880A maps to a region between residue 850 to 900 of human SCY1-like protein 2 using the numbering given in entry Q6P3W7.1 (GeneID 55681).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to SCYL2 immobilized on solid support.

The epitope recognized by A304-880A-T maps to a region between residue 850 to 900 of human SCY1-like protein 2 using the numbering given in entry Q6P3W7.1 (GeneID 55681).

Country of Origin

USA

Additional Product Information

SCY1-like protein 2 (SCYL2) is a component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, it is a probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, it has no detectable kinase activity in vitro. SCYL2 may regulate clathrin-dependent trafficking between the TGN and/or the endosomal system [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6P3W7].

55681

SCYL2

SCY1 like pseudokinase 2

Q6P3W7

SCY1-like protein 2

Alternate Names

coated vesicle-associated kinase of 104 kDa; CVAK104; SCY1-like 2; SCY1-like protein 2; SCY1-like, kinase-like 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.